Journal: iScience
Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility
doi: 10.1016/j.isci.2024.111096
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Article Snippet: Stable clone of adult female human cervical adenocarcinoma HeLa cell line with long telomeres (HeLa1.3 cells); adult male human fibrosarcoma HT1080 cell line overexpressing telomase (HT1080 super-telomerase cells, HT1080-ST) (kindly provided by T. de Lange and J. Lingner, respectively); adult female human osteosarcoma U2OS cell line (ATCC); human embryonic kidney HEK293FT cell line (ATCC); and human female fetal lung fibroblast IMR90 cell line (Coriel Institute) were cultured in DMEM medium supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, F2442) and maintained at 37°C in 5% (v/v) CO 2 .
Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software